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KMID : 0525619980030040303
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1998 Volume.3 No. 4 p.303 ~ p.312
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Isolation and Characterization of Pseudomonas vesicularis KW-15 for Producing Chitinase
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강신욱/Kang SW
정만재/여익현/김동호/Chung MJ/Yeo IH/Kim DH
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Abstract
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A chitinase-producing bacterium was isolated from soil by selective enrichmentment culture medium. The bacterium was identified as synonym of Pseudomonas vesicularis based on the data obtained from its morphological, cultural and biochemical characteristics. The isolated strain was named Pseudomonas vesicularis KW-15. The medium for the production of chitinase was consisted of colloidal chitin 1.0%, yeast extract 1.0%, MgSO4 · 7H2O 0.05%, KH2P04 0.03%, K2HP04 0.07%, NaCl 0.01%, FeSO4 · 7H2O 0.001%, MnCl2 0.0001% and ZnSO4 0.0001%. The optimum cultural temperature, time and initial pH for the best production of chitinase were 30℃, 60hrs and pH 7.0, respectively.
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KEYWORD
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